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rfxcas13d pslq5428  (Addgene inc)


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    Structured Review

    Addgene inc rfxcas13d pslq5428
    Rfxcas13d Pslq5428, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rfxcas13d/pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG+(Plasmid+%23155305)/bio_rxiv__64898__2026__03__14__711150-230-15-17
    Average 92 stars, based on 4 article reviews
    rfxcas13d pslq5428 - by Bioz Stars, 2026-09
    92/100 stars

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    Related Articles

    Amplification:

    Article Title: High-accuracy crRNA array assembly strategy for multiplex CRISPR
    Article Snippet: .. The coding sequences of AsCas12a, VPR, and RfxCas13d were amplified from pY108 (Addgene #84739), pXR001 (#84739), and lenti-EF1a-dCas9-VPR-Puro (#99373) plasmids, respectively. ..

    Article Title: RfxCas13d-mediated inhibition of <i>Circ1647</i> alleviates renal fibrosis via PI3K/AKT signaling pathway
    Article Snippet: .. Initially, Rfxcas13d was amplified from AAV-EFS-NLS-CasRx-U6DR-sgRNA_LacZ-DR (Cat# 154003, Addgene, USA) and integrated into the PX458 vector (Cat# 172221, Addgene, USA) via seamlessly cloning kit (Cat# C115, Vazyme, China). ..

    Expressing:

    Article Title: CRISPR/Cas-mediated mRNA knockdown in the embryos of Xenopus tropicalis.
    Article Snippet: .. The human codon-optimized PspCas13b (pC0046, #103862) and RfxCas13d (pXR001, #109049) expressing plasmids as well as their guide RNA (gRNA) expression plasmids (pC0043, #103854 for PspCas13b; pXR003, #109053 for RfxCas13d) were purchased from Addgene. .. The NLS-RfxCas13d-NLS fragment in pXR001 plasmid was amplified and subcloned into the multiple clone site (MCS) of pcDNA3.1 plasmid (#ZT119) (FENGHUISHENGWU, China) to construct pcDNA3.1-RfxCas13d plasmid.

    Article Title: CRISPR/Cas-mediated mRNA knockdown in the embryos of Xenopus tropicalis
    Article Snippet: .. The human codon-optimized PspCas13b (pC0046, #103862) and RfxCas13d (pXR001, #109049) expressing plasmids as well as their guide RNA (gRNA) expression plasmids (pC0043, #103854 for PspCas13b; pXR003, #109053 for RfxCas13d) were purchased from Addgene. .. The NLS-RfxCas13d-NLS fragment in pXR001 plasmid was amplified and subcloned into the multiple clone site (MCS) of pcDNA3.1 plasmid (#ZT119) (FENGHUISHENGWU, China) to construct pcDNA3.1-RfxCas13d plasmid.

    Article Title: Principles of CRISPR-Cas13 mismatch intolerance enable selective silencing of point-mutated oncogenic RNA with single-base precision.
    Article Snippet: .. For 96- well plates, master mix A contained 25 ng of the Cas13 effector plasmid [PspCas13b- NES- 3xFLAG- T2A- BFP (Addgene #173029) or pLentiRNACRISPR- 007- RfxCas13d (Addgene #138149)], target plasmid (50 ng of BRAF or 100 ng of KRAS expression plasmids), crRNA plasmid (0.1 to 100 ng), and 4% (v/v) P3000 enhancer reagent (Invitrogen) in Opti- MEM serum- free medium (Gibco), making up 5- μl total volume for 96- well plates. .. Master mix B contained 6% (v/v) Lipofectamine 3000 reagent (Invitrogen) in Opti- MEM serum- free medium, making up 5- μl total volume.

    Plasmid Preparation:

    Article Title: Local anesthetic tetracaine hydrochloride induces pyroptosis via caspase-3/gasdermin E in uveal melanoma.
    Article Snippet: Background: Evasion of pyroptosis is an effective survival strategy employed by cancer cells to evade immune cell attacks and drug-induced cytotoxicity.. Exploring potent molecules capable of inducing pyroptosis in cancer cells has significant clinical implications for the control of cancer progression.. Unexpectedly, we found that the local anesthetic tetracaine hydrochloride (TTC) induced pyroptosis, specifically in uveal melanoma but not in acral or

    Article Title: Principles of CRISPR-Cas13 mismatch intolerance enable selective silencing of point-mutated oncogenic RNA with single-base precision.
    Article Snippet: .. For 96- well plates, master mix A contained 25 ng of the Cas13 effector plasmid [PspCas13b- NES- 3xFLAG- T2A- BFP (Addgene #173029) or pLentiRNACRISPR- 007- RfxCas13d (Addgene #138149)], target plasmid (50 ng of BRAF or 100 ng of KRAS expression plasmids), crRNA plasmid (0.1 to 100 ng), and 4% (v/v) P3000 enhancer reagent (Invitrogen) in Opti- MEM serum- free medium (Gibco), making up 5- μl total volume for 96- well plates. .. Master mix B contained 6% (v/v) Lipofectamine 3000 reagent (Invitrogen) in Opti- MEM serum- free medium, making up 5- μl total volume.

    Knockdown:

    Article Title: Local anesthetic tetracaine hydrochloride induces pyroptosis via caspase-3/gasdermin E in uveal melanoma.
    Article Snippet: Background: Evasion of pyroptosis is an effective survival strategy employed by cancer cells to evade immune cell attacks and drug-induced cytotoxicity.. Exploring potent molecules capable of inducing pyroptosis in cancer cells has significant clinical implications for the control of cancer progression.. Unexpectedly, we found that the local anesthetic tetracaine hydrochloride (TTC) induced pyroptosis, specifically in uveal melanoma but not in acral or


    other:

    Article Title: Multimodal control of Cas13 activity through domain insertion at an allosteric hotspot
    Article Snippet: Plasmids associated with Mu transposition were originally obtained as a gift from David Savage which include pUCKanR-Mu-BsaI (Addgene #79769) and pATT-Dest (Addgene #79770) .

    Bioprocessing:

    Article Title: RfxCas13d-mediated inhibition of <i>Circ1647</i> alleviates renal fibrosis via PI3K/AKT signaling pathway
    Article Snippet: .. Initially, Rfxcas13d was amplified from AAV-EFS-NLS-CasRx-U6DR-sgRNA_LacZ-DR (Cat# 154003, Addgene, USA) and integrated into the PX458 vector (Cat# 172221, Addgene, USA) via seamlessly cloning kit (Cat# C115, Vazyme, China). ..

    Cloning:

    Article Title: RfxCas13d-mediated inhibition of <i>Circ1647</i> alleviates renal fibrosis via PI3K/AKT signaling pathway
    Article Snippet: .. Initially, Rfxcas13d was amplified from AAV-EFS-NLS-CasRx-U6DR-sgRNA_LacZ-DR (Cat# 154003, Addgene, USA) and integrated into the PX458 vector (Cat# 172221, Addgene, USA) via seamlessly cloning kit (Cat# C115, Vazyme, China). ..

    CRISPR:


    Construct:




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